Nucleoside triphosphate regeneration decreases the frequency of translation errors.

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Nucleoside triphosphate regeneration decreases the frequency of translation errors.

The addition of naturally occurring polyamines and inorganic ions to an in vitro protein-synthesizing system improved the extent and fidelity of translation. In such an optimized system, regeneration of the nucleoside triphosphates with phosphoenolpyruvate and pyruvate kinase (ATP:pyruvate 2-O-phosphotransferase, EC 2.7.1.40) reduced further the missense error frequency to the in vivo level as ...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1979

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.76.7.3174